RAPID COMMUNICATION Transduction of Primitive Human Hematopoietic Cells With Recombinant Adenovirus Vectors

Transduction of primitive human hematopoietic cells with recombinant adenovirus vectors.

We have examined the ability of recombinant adenoviral vectors to transduce human hematopoietic cells. Our findings indicate that adenovirus readily infects a large proportion of CD34+ cells. Using adenovirus vectors that transduce either a lacZ or an alkaline phosphatase reporter gene, we observed up to 45% of total CD34+ cells infected. Upon more detailed analysis, we observed comparable leve...

Influence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells

Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

Redirected infection of directly biotinylated recombinant adenovirus vectors through cell surface receptors and antigens.

The inability of adenovirus to infect primitive hematopoietic cells presents an obstacle to the use of adenovirus vectors for gene transfer to these cell types. Therefore, expanding the tropism of adenovirus vectors to unique cell surface antigens would be an important development for gene therapy protocols. In this study, we sought to redirect infection of adenovirus vectors to primitive human...

Acute lymphoblastic leukemia/lymphoblastic lymphoma of natural killer (NK) lineage: quest for another NK-lineage neoplasm.

Joanne M. Wroblewski lez-Garcia M, Koedens M, Fukunga N, Davidson B, Dick J, Minn A, Boise L, Thompson C, Wicha M, Nunez G: A recombinant bclL. Travis Lay xs adenovirus selectively induces apoptosis in cancer cells but not Timothy C. Meeker in normal bone marrow cells. Proc Natl Acad Sci USA 92:11024, Markey Cancer Center 1995 University of Kentucky 4. Wroblewski J, Lay L, Van Zant G, Phillips ...

Repository of the Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association http://edoc.mdc-berlin.de/15951 In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectors